Composite

Part:BBa_K2957038

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-20)


hEF1a, EYFP

Contents: hEF1a, EYFP, SynthpA, with Amp resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and EYFP reporter gene. It is another version of BBa_K2957084 (pIG_030).

What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid. If you would like the complete correct sequence please contact igem-2019-students@mit.edu and igem-2019-mentors@mit.edu

How to use it?: This part was used for transfection into HEKs. See more on the Parts Overview Page or MIT iGEM 2019 wiki: (a href=https://2019.igem.org/Team:MIT/Parts)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1013
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1013
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1013
    Illegal AgeI site found at 81
    Illegal AgeI site found at 1019
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None